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Citrate buffer pH 6.0. Slides were left to cool down in the antigen retrieval solution for 20 minutes. Endogenous peroxidase activity was quenched by first incubating the specimens for 5 min in 3 hydrogen peroxide (supplied by user). The specimens were then incubated with the primary antibody against Apo-J (clone sc-6420, goat polyclonal antibody, Santa Cruz Biotechnology Inc, CA) in a dilution 1
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Internal carotid artery endarterectomy using synthetic polytetrafluoroethylene (PTFE) patch (W.L.Gore Associates, Newark, Delaware) under general anesthesia. Shunt for maintaining brain blood flow was not used in these operations. After endarterectomy, carotid plaque was carefully placed in 10 buffered formalin for further processing. Postoperatively, patients were normally recovered and left h
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Ean RT, Brown AJ: Apolipoprotein J (clusterin) induces cholesterol export from macrophage-foam cells: a potential anti-atherogenic function? Biochem J 1998, 331(1):231?37. 4. Ahuja HS, Tenniswood M, Lockshin R, Zakeri ZF: Expression of clusterin in cell differentiation and cell death. Biochem Cell Biol 1994, 72(11?2):523?30. 5. Poon S, Easterbrook-Smith SB, Rybchyn MS, Carver JA, Wilson MR: Cluste
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Ean RT, Brown AJ: Apolipoprotein J (clusterin) induces cholesterol export from macrophage-foam cells: a potential anti-atherogenic function? Biochem J 1998, 331(1):231?37. 4. Ahuja HS, Tenniswood M, Lockshin R, Zakeri ZF: Expression of clusterin in cell differentiation and cell death. Biochem Cell Biol 1994, 72(11?2):523?30. 5. Poon S, Easterbrook-Smith SB, Rybchyn MS, Carver JA, Wilson MR: Cluste
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E. 4Laboratory of Experimental Surgery and Surgery Research, Medical School, National and Kapodistrian University of Athens, 15B Ag Thoma Str, Goudi, Athens, Greece. Received: 9 March 2014 Accepted: 14 April 2014 Published: 23 April 2014 References 1. Jenne DE, Tschopp J: Clusterin: the intriguing guises of a widely expressed glycoprotein. Trends Biochem Sci 1992, 17(4):154?59. 2. de Silva HV, Stu
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E. 4Laboratory of Experimental Surgery and Surgery Research, Medical School, National and Kapodistrian University of Athens, 15B Ag Thoma Str, Goudi, Athens, Greece. Received: 9 March 2014 Accepted: 14 April 2014 Published: 23 April 2014 References 1. Jenne DE, Tschopp J: Clusterin: the intriguing guises of a widely expressed glycoprotein. Trends Biochem Sci 1992, 17(4):154?59. 2. de Silva HV, Stu
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Sitive family history of confirmed cardiovascular diseases considering first degree relatives was completed. Hypertension, when the patient was not taking any medication, was defined as systolic blood pressure 140 mmHg and/or diastolic blood pressure 90 mmHg and diabetes mellitus as two fasting plasma glucose levels of 126 mg/dL or higher. Patient medication was recorded in detail and categorized
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Sitive family history of confirmed cardiovascular diseases considering first degree relatives was completed. Hypertension, when the patient was not taking any medication, was defined as systolic blood pressure 140 mmHg and/or diastolic blood pressure 90 mmHg and diabetes mellitus as two fasting plasma glucose levels of 126 mg/dL or higher. Patient medication was recorded in detail and categorized
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M/content/13/1/Page 6 ofAHThCHTh**TXNIP -actinBTDTTXNIP -actin***Figure 3 TXNIP overexpression in ATC HTh74 and T238 cells attenuates glucose uptake. HTh74 and T238 cells were transduced with retrovirus encoding human TXNIP or vector control as well as a selectable antibiotic resistance marker, and stable pools were generated under antibiotic selection. Western blot analysis of whole cell lysates
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Gnosis and increased mortality [49,50]. The mechanism underlying this differential glucose uptake between well-differentiated PTC and ATC is not well understood. The novel finding that TXNIP expression is low in ATC is consistent with the observed FDG uptake on PET/CT in patients with ATC, supporting a critical role for TXNIP as a metabolic regulator in thyroid cancer progression. In addition to i